Which of the following options is used to create assembly constraints in Autodesk Inventor?

The Insert Constraint tool is designed specifically for establishing assembly constraints in Autodesk Inventor. This tool allows users to define how components interact and relate to one another within an assembly. By using this tool, users can set parameters such as distance, angle, and alignment between parts, which is essential for achieving a properly constrained assembly that behaves as intended in simulations and during physical assembly.

The other options serve different purposes. The Align tool is primarily used to position components in a specific orientation rather than to define complex relationships. The Pin Tool is typically for fixing parts in a specific location without allowing movement, while the Joint command is used for creating joints that simulate motion, such as rotation or sliding, between parts rather than standard constraints. The focus of the correct answer is on the foundational functionality of constraining components in a manner that is essential for any assembly design process in Autodesk Inventor.